Protective effects of cyanidin-3-rutinoside against monosaccharides-induced protein glycation and oxidationInternational Journal of Biological Macromolecules


Thavaree Thilavech, Sathaporn Ngamukote, Mahinda Abeywardena, Sirichai Adisakwattana
Molecular Biology / Structural Biology / Biochemistry


Protective Effects of Cyanidin-3-O-β-glucopyranoside Against UVA-induced Oxidative Stress in Human Keratinocytes¶

Andrea Tarozzi, Alessandra Marchesl, Silvana Hrelia, Cristina Angeloni, Vincenza Andrisano, Jessica Fiori, Giorglo Cantelli-Forti, Patrizia Hrella

Protective effect of cyanidin-3- O -β-D-glucopyranoside fraction from mulberry fruit pigment against oxidative damage in streptozotocin-induced diabetic rat bladder

U-Syn Ha, Woong-Jin Bae, Su-Jin Kim, Byung-Il Yoon, Hoon Jang, Sung-Hoo Hong, Ji-Yeoul Lee, Seung-Yeon Hwang, Sae-Woong Kim

Inhibitory effect of Clitoria ternatea flower petal extract on fructose-induced protein glycation and oxidation-dependent damages to albumin in vitro

Poramin Chayaratanasin, Manuel Barbieri, Nipattra Suanpairintr, Sirichai Adisakwattana


International Journal of Biological Macromolecules 75 (2015) 515–520

Contents lists available at ScienceDirect

International Journal of Biological Macromolecules j ourna l h o mepa ge: www.elsev ier .com/ locate / i jb iomac

Protective effects of cyanidin-3-rutinoside agains monos nd

Thavaree Abe

Sirichai A a Program in Bi nd b Research Gro korn U c Department o angko d CSIRO Food& a r t i c l

Article history:

Received 6 No

Received in re

Accepted 4 Feb

Available onlin



Advanced glycation end products

Monosaccharide ally o we nt pr d pro albumin (BSA).The results demonstrated that C3R (0.125–1.00 mM) inhibited the formation of fluorescent AGEs in ribose-glycated BSA (2–52%), fructose-glycated BSA (81–93%), glucose-glycated BSA (30–74%) and galactose-glycated BSA (6-79%).Correspondingly, C3R (1.00 mM) decreased the level of

Nε-(carboxymethyl) lysine (56-86%) in monosaccharide-induced glycation in BSA. C3R also reduced the level of fructosamine, -amyloid cross structure, protein carbonyl content as well as the depletion of thiol in BSA/monosaccharide system. In summary, C3R might offer a new promising antiglycation agent for 1. Introdu

The rapi global publ groups wor 4.4% in 203 and type 2 role in the effects are stances call from a non group of re proteins, co glycation is vascular co ∗ Correspon

Health Science

Tel.: +662 218

E-mail add http://dx.doi.o 0141-8130/© the prevention of diabetic complications by inhibiting AGE formation and oxidation-dependent protein damage. © 2015 Elsevier B.V. All rights reserved. ction d growing burden of diabetes has become a serious ic health issue. The prevalence of diabetes for all ageldwide has been estimated to be 2.8% in 2000 and 0 [1]. It is now well recognized that in both type 1 diabetes chronic hyperglycemia plays an important pathology of vascular damage [2,3]. Its deleterious attributable to the formation of sugar-derived subed advanced glycation end products (AGEs). AGEs result -enzymatic glycation reaction between the carbonyl ducing monosaccharides and the free amino group of mmonly described as protein glycation [4,5]. Protein particularly known to underlie the macro and micromplications in diabetes. ding author at: Department of Nutrition and Dietetics, Faculty of Allied s, Chulalongkorn University, Bangkok 10330, Thailand. 1067; fax: +662 218 1076. ress: (S. Adisakwattana).

The receptor for AGEs (RAGE), first described as a signal transduction receptor for AGEs, has now been identified as a multi-ligand receptor that is able to bind a number of agents including AGEs, high mobility group protein (B)1, S-100 calcium-binding protein, phosphatidyl serine as well as amyloid--protein and -sheet fibrils [6–8]. The interaction of AGEs with RAGE triggers the overproduction of free radicals and promotes inflammation through activation of the MAPK pathway leading to diabetic complications [8]. Indeed, emerging evidence reveals that increased serum level of AGEs is well correlated with the development of vascular complications in type 2 diabetic patients [9,10].

There has been considerable interest in the types of dietary monosaccharides for induction of protein glycation. An aldohexose (glucose and galactose) as well as a ketohexose (fructose) are a type of simple monosaccharide found in plants, fruits, and foods. Aside from hexose sugars that play a vital nutritional role, an aldopentose (ribose) can be obtained from diet, especially from foods containing high content of riboflavin such as cereal, meat and dark-green vegetable. In addition, ribose is endogenously synthesized from glucose through the hexose monophosphate shunt [11]. The rate of protein glycation-mediated by monosaccharides varies in their reactiveness which depends on the rg/10.1016/j.ijbiomac.2015.02.004 2015 Elsevier B.V. All rights reserved.accharides-induced protein glycation a

Thilavecha,b, Sathaporn Ngamukoteb,c, Mahinda disakwattanab,c,∗ omedical Sciences, Graduate School, Chulalongkorn University, Bangkok 10330, Thaila up of Herbal Medicine for Prevention and Therapeutic of Metabolic Diseases, Chulalong f Nutrition and Dietetics, Faculty of Allied Health Sciences, Chulalongkorn University, B

Nutrition Flagship, Adelaide SA 5000, Australia e i n f o vember 2014 vised form 3 February 2015 ruary 2015 e 12 February 2015 a b s t r a c t

Cyanidin-3-rutinoside (C3R), a natur etables as a colorant. C3R has been activities attributed to its antioxida

C3R against monosaccharide-inducet oxidation ywardenad, niversity, Bangkok 10330, Thailand k 10330, Thailand ccurring anthocyanin, is present in various fruits and vegll characterized and demonstrated a number of biological operties. The present study compared the effectiveness of tein glycation and oxidation in vitro using bovine serum 516 T. Thilavech et al. / International Journal of Biological Macromolecules 75 (2015) 515–520

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