Cadaverine Production By Heterologous Expression of Klebsiella Oxytoca Lysine Decarboxylase

Process for the fermentative production of 1,5-diaminopentane

The present invention relates to a method for the isolation of 1,5-diaminopentane (DAP) from DAP-containing fermentation broths, a method for the fermentative production of DAP using this isolation method and a method for the production of DAP-containing polymers using the in DAPs isolated or fermentatively produced in this way.


1,5-diaminopentane (often also referred to as pentamethylenediamine or cadaverine; hereinafter referred to as DAP) is an important raw material in the chemical industry. For example, DAP is used in the production of polyamides, polyureas or polyurethanes and copolymers from them.

In addition, the fermentative or enzymatic production of DAP by decarboxylation of lysine has been known for a long time. Various processes for isolating the product of value from the fermentation broth are described.

For example, EP-A-1 482 055 describes the enzymatic decarboxylation of lysine in the presence of a dicarboxylic acid to adjust the pH during the reaction. The DAP dicarboxylate obtained during production is isolated by first decolorizing the valuable solution with activated carbon, concentrating it and crystallizing the DAP dicarboxylate by means of cooling crystallization.

WO-A-2006/123778 describes the production of DAP carbonate by enzymatic decarboxylation of lysine in the presence of carbon dioxide. DAP is formed by concentrating the reaction solution and splitting off carbon dioxide.

JP 2004-208 646 describes the production of DAP dicarboxylate by enzymatic decarboxylation of a solution containing L-lysine dicarboxylate and precipitation of DAP dicarboxylate by adding an organic solvent selected from alcohols, ketones and nitriles.

JP 2004-222 569 describes the production of DAP using an L-lysine decarboxylase-expressing coryneform bacterium, adjustment of the culture supernatant to pH 12 and extraction of DAP with a polar organic solvent.

Finally, JP 2004-000 114 describes the production of DAP by reacting highly concentrated L-lysine monohydrochloride with L-lysine decarboxylase-expressing E. co // cells, adjusting the reaction solution to pH ≥13 and extracting the reaction product with a polar organic solvent and subsequent distillation.

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