A Comprehensive Study of Extraction of L-Lysine with Sec -Octylphenoxy Acetic Acid in Sulfonated KeroseneSeparation Science and Technology

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Authors
Jie Zhang, Xiaohua Zhou, Dan Wang, Xing Zhou, Fang Yun, Shiyu Tan
Year
2014
DOI
10.1080/01496395.2013.876047
Subject
Chemistry (all) / Filtration and Separation / Process Chemistry and Technology / Chemical Engineering (all)

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A Comprehensive Study of Extraction of L-Lysine with

Sec-Octylphenoxy Acetic Acid in Sulfonated Kerosene

Jie Zhanga, Xiaohua Zhoua, Dan Wanga, Xing Zhoub, Fang Yunc & Shiyu Tana a College of Chemistry and Chemical Engineering, Chongqing University, Chongqing, China b TCM Pharmacochemistry Institute, Chongqing Academy of Chinese Materia Medica,

Chongqing, China c Chongqing Minfeng Chemical Co., Ltd, Chongqing, China

Accepted author version posted online: 07 Mar 2014.Published online: 03 Jun 2014.

To cite this article: Jie Zhang, Xiaohua Zhou, Dan Wang, Xing Zhou, Fang Yun & Shiyu Tan (2014) A Comprehensive Study of

Extraction of L-Lysine with Sec-Octylphenoxy Acetic Acid in Sulfonated Kerosene, Separation Science and Technology, 49:9, 1349-1356, DOI: 10.1080/01496395.2013.876047

To link to this article: http://dx.doi.org/10.1080/01496395.2013.876047

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Separation Science and Technology, 49: 1349–1356, 2014

Copyright © Taylor & Francis Group, LLC

ISSN: 0149-6395 print / 1520-5754 online

DOI: 10.1080/01496395.2013.876047

A Comprehensive Study of Extraction of L-Lysine with

Sec-Octylphenoxy Acetic Acid in Sulfonated Kerosene

Jie Zhang,1 Xiaohua Zhou,1 Dan Wang,1 Xing Zhou,2 Fang Yun,3 and Shiyu Tan1 1College of Chemistry and Chemical Engineering, Chongqing University, Chongqing, China 2TCM Pharmacochemistry Institute, Chongqing Academy of Chinese Materia Medica, Chongqing, China 3Chongqing Minfeng Chemical Co., Ltd, Chongqing, China

Extraction of L-lysine with sec-octylphenoxy acetic acid (CA-12) in sulfonated kerosene was investigated under different pH and CA-12 concentrations, and optimized conditions were determined. Half-saturation fluorometric analysis and atomic force microscopy showed that the electrovalent bond between

L-lysine and CA-12 acted as the main force to partition L-lysine into organic phase. Moreover, the mathematically analyzed extraction process indicated that the extractive complex was a 1:2 L-lysine • CA-12 structure. The initial extraction efficiency could reach 78.9 ± 1.2% and the total strip extraction efficiency after three strip extractions could reach 95.7 ± 1.8% by using a dilute KCl solution.

Keywords sec-octylphenoxy acetic acid; L-lysine; extraction; atomic force microscopy; fluorescence

INTRODUCTION

Amino acids are widely used in feed, food, and the medicine industry. Out of the twenty naturally occurring amino acids,

L-Lysine (Lys) is one of the 9 essential and commercially important amino acids, ecumenically found in naturally occurring proteins of all living organisms. Lys is often used as special chemical in medicament, chemical agent, food material, and feed additive (1-3). Recently, its production and purification technology have attracted great attention. Lys is largely produced by microbial fermentation nowadays (4, 5). The downstream separation and purification is a very important factor enormously affecting process effectiveness and production costs, which is steadily requesting improvements in the recovery process. After cell separation by cell filtration

Received 14 May 2013; accepted 9 December 2013.

Address correspondence to Dan Wang, College of Chemistry and

Chemical Engineering, Chongqing University, Chongqing, China.

E-mail: danwang088@gmail.com

Color versions of one or more of the figures in the article can be found online at www.taylorandfrancis.com/lsst. or centrifugation, Lys may be recovered from fermentation broth by an ion exchange step and thereafter concentrated by evaporation and spray drying (6, 7). However, this ion exchange step is complicated and costly, and a great deal of waste water is generated during the regeneration of ion-exchange resins (8). To make the separation and purification of Lys more costeffective and efficient, it is necessary to develop new methods for recovery and purification.

Liquid-liquid extraction is widely used for separation and purification of solutes from fermentation broth, industrial wastes, and other industrial streams due to its multiple advantages of excellent efficiency, large production capacity, reusable extract, and ease of operation and automation (9–12). Recently, solvent extraction technology, which is used for separation of organic acids from aqueous solutions, appeals to many researchers. Lin et al. found that selective separation of phenylalanine from binary mixture of phenylalanine and aspartic acid was possible with cationic extractant when they were extracted at pH 5.0 and stripped using 0.55 mol/L HCl solution (13). Oshite et al. found that tryptophan-fluorescamine was selective for the extraction using the homogeneous liquidliquid extraction method with perfluorooctanoic acid (HPFOA) (14). Tomé et al. have successfully adopted hydrophobic ionic liquids (ILs) as solvents for tryptophan liquid-liquid extraction processes (15). Schuur et al. investigated the continuous enantioselective liquid-liquid extraction of aqueous 3,5-dinitrobenzoyl-(R),(S)-leucine (AR, S) using O-(1-tbutylcarbamoyl)- 11-octadecylsulfinyl- 10,11-dihydro-quinine (C, a cinchona alkaloid) as extractant in 1,2- dichloroethane (DCE) (16). In Wu’s study, the low concentration Lys was separated from the aqueous system by solvent sublation method with the anionic surfactant (DBSA) and the extractant (di(2-ethylhexyl) phosphoric acid, D2EHPA) (8). The extractant